IRAK4 Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC203041 and based on accession number NM_016123. The protein contains a C-terminal DDK tag.
|
| Specificity |
Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 2, mRNA.
|
| Gene |
IRAK4
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Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
|
| Theoretical MW |
51.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
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| Buffer |
RIPA buffer
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Lysate Details for IRAK4
| Type |
Overexpression
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| Protein State |
Native
|
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for IRAK4 Overexpression Lysate (Native)
- EC 2.7.11
- EC 2.7.11.1
- interleukin-1 receptor-associated kinase 4
- IPD1
- IRAK4
- IRAK-4
- NY-REN-64
- REN64
- Renal carcinoma antigen NY-REN-64
Background
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq]. Transcript Variant: This variant (2) differs in the 5 UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.