Product: Sodium lauryl polyoxyethylene ether sulfate
CCR1 Antibody Summary
| Immunogen |
Synthetic peptide taken within amino acid region 1-50 on mouse CCR1 protein.
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| Specificity |
The antibody does not cross reacts with other members of the CCR family.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
CCR1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
NBP1-78173 is ideal for WB, IHC, Dot Blot and ELISA applications. This antibodie do not cross react to other chemokine receptors.
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| Publications |
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Reactivity Notes
The CCR1 peptides sequences were conserved in rat, mouse and human protein and also present in other species.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.65-0.75 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for CCR1 Antibody
- C-C chemokine receptor type 1
- CC-CKR-1
- CCR1
- CCR-1
- CD191 antigen
- CD191
- chemokine (C-C motif) receptor 1
- CKR1
- CKR-1
- CMKBR1SCYAR1
- CMKR1
- HM145C-C CKR-1
- LD78 receptor
- Macrophage inflammatory protein 1-alpha receptor
- MIP-1alpha-R
- MIP1aR
- RANTES receptor
- RANTES-R
Background
Chemokine receptors represent a subfamily of ~20 GPCRs that were originally identified by their roles in immune cell trafficking. Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and RANTES, members of the beta chemokine family of leukocyte chemo-attractants, bind to a common seven-transmembrane-domain human receptor. Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CCR) belong to the superfamily of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and directing the proliferation and migration of immature neurons, glia and their precursors (1). Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases. Induction of chemokines and infiltration of chemokine receptor-bearing cells has also been shown in a variety of animal models of renal diseases, as well as in human diseases and allograft rejection (2) Activation of naive T cells by specific antigen and cytokines, up-regulate cell adhesion molecules (CAM) as well as chemokine receptors on their surface, which directs them to migrate towards the inflamed tissues. Several subtypes of CCR have been characterized (CCR1-CCR9). The CCR1 receptors are activated by its ligand CCL5/RANTES and control cell migration associated with routine immune surveillance, inflammation and development. CCR1 also plays important role in normal physiology, cancer and AIDS, thus making them important therapeutic targets. The CCR1 receptor protein is approximately 45 kDa (355 amino acids) protein which has 7 transmembrane domains, characteristic of G-protein coupled receptors. The CCR4 receptors are activated by Thymus and activation-regulated chemokine (TARC) that is expressed constitutively in thymus and transiently in stimulated peripheral blood mononuclear cells. The protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins. The short carboxy terminal is intracellular and has putative post-translational sites. The Anti-CCR1-selective antibodies were generated against conserved but unique sequences from human and mouse CCR1 proteins. These sequences reside near the N-terminal end of CCR1 and are unique to CCR1 protein. The antibodies to CCR1 are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. The affinity purified antibodies are also conjugated to FITC and biotin for direct applications in IHC and cell sorting experiments.