Histone H3 [ac Lys9] Antibody (AH3-120)

Product: PF-915275

Histone H3 [ac Lys9] Antibody (AH3-120) Summary

Immunogen
A synthetic, acetylated histone H3 peptide (amino acids 7-20, acetyl K9) corresponding to the N-terminus of human histone H3, conjugated to KLH.
Modification
ac Lys9
Localization
Nuclear
Specificity
Staining of (acetyl K9) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HIST3H3
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
WB: Detects a band of approximately 17 kDa. (Determined using a whole cell extract of mouse fibroblasts 3T3 cell line treated with sodium butyrate.) We advise you to centrifuge this product vial before use. ELISA: indirect.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Mouse, Rat, Xenopus laevis, Cow, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to very high or perfect sequence identity across many species. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
2.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Histone H3 [ac Lys9] Antibody (AH3-120)

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3K9ac
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

PMID: 2322828