Product: PF-CBP1 (hydrochloride)
Histone H3 [ac Lys9, p Ser10] Antibody Summary
| Immunogen |
Synthetic acetylated and phosphorylated [Ac-Lys9, pSer10] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.
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| Modification |
ac Lys9, p Ser10
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| Specificity |
Synthetic acetylated and phosphorylated [Ac-Lys9, pSer10] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3. Staining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
HIST3H3
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| Purity |
IgG purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
ChIP:Approximately 4-5 ug of each antibody was used with 30-40 ug of chromatin per ChIP.IF: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Detects a band of approximately 17 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.We advise you to centrifuge this product vial before use.
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Reactivity Notes
Cross-reacts with Human, Bovine, Drosophila, Mouse, Rat and Chicken.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
10mM PBS
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| Preservative |
15mM Sodium Azide
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| Purity |
IgG purified
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Alternate Names for Histone H3 [ac Lys9, p Ser10] Antibody
- H3 histone, family 3A
- H3.3AH3F3H3F3B
- H3.3B
- H3F3A
- H3K9ac
- Histone H3
- histone H3.3
- MGC87782
- MGC87783
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination, are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by the modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Phosphorylation, particularly that of histones H1 and H3, has long been implicated in chromosome condensation during mitosis. However, converging evidence suggests that H3 phosphorylation (specifically serine 10) is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. The potential importance of the serine 10 phosphorylation mark in H3 is strengthened by the finding that MSK1, a kinase activated by growth factor and stress stimuli, also phosphorylates H3 in vitro. H3 phosphorylation at serine 10 in conjunction with phosphorylation at serine 28, is also required for proper segregation and condensation of chromosomes during mitosis and meiosis.