NGFRAP1/BEX3/NADE Antibody Summary
| Immunogen |
Antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE. The immunogen is located within the last 50 amino acids of NADE.
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
NGFRAP1
|
| Purity |
Ion exchange chromatography
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
||
| Application Notes |
This antibody can be used for detection of NADE by Western blot at 0.5 to 2 mg/ml. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE.
|
||
| Control Peptide |
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Ion exchange chromatography
|
Alternate Names for NGFRAP1/BEX3/NADE Antibody
- BEX3
- BEX3p75NTR-associated cell death executor
- Brain-expressed X-linked protein 3
- DXS6984EX-linked 3
- HGR74
- NADE
- nerve growth factor receptor (TNFRSF16) associated protein 1
- Nerve growth factor receptor-associated protein 1
- NGFRAP1
- Ovarian granulosa cell 13.0 kDa protein HGR74
- ovarian granulosa cell protein (13kD)
- protein BEX3
Background
The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.