Enolase 2/Neuron-specific Enolase Antibody Summary
| Immunogen |
Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.
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| Localization |
Cytoplasmic
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| Marker |
Neuronal Marker
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| Specificity |
This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. It gives a specific staining in neurons of the brain, islets of Langerhans in pancreas, and in peripheral nerves of the skin. No staining with other tissue types has been seen.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
ENO2
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| Purity |
Unpurified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
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| Positive Control |
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Reactivity Notes
Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
Whole antisera
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| Preservative |
0.1% Sodium Azide
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| Purity |
Unpurified
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Notes
This product is available in a small concetrated size as well as a larger pre-dilute size. The larger can be used neat without further dilution.
Alternate Names for Enolase 2/Neuron-specific Enolase Antibody
- 2-phospho-D-glycerate hydrolyase
- 2-phospho-D-glycerate hydro-lyase
- EC 4.2.1.11
- ENO2
- enolase 2 (gamma, neuronal)
- Enolase 2
- gamma-Enolase
- Neural enolase
- neuron specific gamma enolase
- neurone-specific enolase
- Neuronspecific Enolase
- Neuron-specific enolase
- NSE
Background
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.