PA28 Activator beta Subunit/PSME2 Antibody Summary
| Immunogen |
Synthetic Peptide: M(1) A K P C G V R L S G E A R(14)
|
| Specificity |
Detects recombinant human proteasome 11S REG beta. This does not detect endogenous levels of proteasome 11S REG beta nor does it detect other 11S REG subunits.
|
| Isotype |
IgG
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
PSME2
|
| Purity |
Ammonium sulfate precipitation
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
WB: Detects an approx. 28 kDa protein representing recombinant human proteasome 11S REG beta.
|
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Ammonium sulfate precipitation
|
Alternate Names for PA28 Activator beta Subunit/PSME2 Antibody
- 11S regulator complex beta subunit
- 11S regulator complex subunit beta
- Activator of multicatalytic protease subunit 2
- MCP Activator
- MCP activator, 31-kD subunit
- PA28 Activator beta Subunit
- PA28b
- PA28betacell migration-inducing protein 22
- proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
- Proteasome activator 28 subunit beta
- proteasome activator 28-beta
- proteasome activator complex subunit 2
- proteasome activator hPA28 subunit beta
- PSME2
- REGbeta
- REG-beta
Background
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelmans syndrome, and Liddle syndrome.