VEGF R2/KDR/Flk-1 Antibody (MM0002-2F66) Summary
| Immunogen |
This antibody was produced from a hybridoma (mouse myeloma fused withspleen cells from a mouse immunized with human VEGFR2 N-terminal recombinant protein.
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| Marker |
Endothelial Cell Marker
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| Specificity |
VEGFR2. This antibody shows no cross-reactivity with hVEGFR1, hVEGFR3.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
KDR
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| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
In western blots, this antibody shows no cross-reactivity with hVEGFR1, hVEGFR3.
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| Positive Control |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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| Buffer |
A 0.2 um filtered solution in PBS
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| Preservative |
No Preservative
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| Concentration |
LYOPH
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| Purity |
Protein G purified
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| Reconstitution Instructions |
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.
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Alternate Names for VEGF R2/KDR/Flk-1 Antibody (MM0002-2F66)
- CD309 antigen
- CD309
- EC 2.7.10
- EC 2.7.10.1
- Fetal liver kinase 1
- fetal liver kinase-1
- Flk1
- Flk-1
- FLK1tyrosine kinase growth factor receptor
- KDR
- kinase insert domain receptor (a type III receptor tyrosine kinase)
- Kinase insert domain receptor
- Protein-tyrosine kinase receptor flk-1
- soluble VEGFR2
- vascular endothelial growth factor receptor 2
- VEGF R2
- VEGFR
- VEGFR2
- VEGFR-2
Background
VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.