AU5 Epitope Tag Antibody Summary
| Immunogen |
Rabbits were immunized with AU5 (TDFYLK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
ELISA 1:1,000 – 1:30,000; for coating plates 1:100 – 1:500
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
PBS
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for AU5 Epitope Tag Antibody
- AU5 epitope tag
- TDFYLK epitope tag
- TDFYLK tag
Background
AU5 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. The recognized AU5 epitope represents the amino acid sequence TDFYLK.