RC74 Antibody Summary
| Immunogen |
A synthetic peptide that maps to a region between residues 350 and 400 of human Related to CPSF subunits 74 kDa using the numbering given in TrEMBL entry Q8TB16 (GeneID 55756).
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| Specificity |
NB100-590 is specific for human RC74 protein.
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| Predicted Species |
Mouse (100%), Rat (100%), Primate (100%), Guinea Pig (100%), Monkey (100%), Zebrafish (100%), Rabbit (100%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
INTS9
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: Not recommended. Use 100-589 for WB Immunoprecipitation: 1-4 ug/mg lysate Histo/cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
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| Positive Control |
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Reactivity Notes
Based on 100% sequence identity, this antibody is predicted to react Orangutan, Rhesus Monkey, Chimpanzee, West Indian ocean coelacanth, White-tufted-ear marmoset, Crab-eating macaque, Chinese hamster, Naked mole rat and Spotted green pufferfish.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for RC74 Antibody
- CPSF2L
- FLJ10871
- INT9
- integrator complex subunit 9
- Protein related to CPSF subunits of 74 kDa
- RC74
- RC-74
Background
Formation of the mature 3 ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing, among other proteins, CPSF-73 and CPSF-100. RC-74 is an exclusively nuclear protein found in HeLa cells that has similar characteristics to CPSF-100. Like the complex that forms between CPSF-100 and CPSF-73, studies show that RC-74 may form a similar complex with RC-68 that is independent from the CPSF complex. This RC-68 / RC-74 complex may be involved in a pre-mRNA processing event other than cleavage and polyadenylation, such as the 3 end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase.