ROCK2 Antibody Summary
| Immunogen |
The immunogen recognized by this antibody maps to a region between residues 1 and 100 of human Rho-associated, coiled-coil containing protein kinase 2 using the number given in entry NP_004841.1 (GeneID 9475).
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| Specificity |
NB100-592 is specific for human Rho Kinase alpha protein.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
ROCK2
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 – 1:25,000 Immunoprecipitation: 2 to 5 ug/mg of lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
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| Positive Control |
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| Publications |
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Reactivity Notes
Human
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for ROCK2 Antibody
- coiled-coil-containing protein kinase 2
- EC 2.7.11
- EC 2.7.11.1
- KIAA0619
- p164 ROCK-2
- Rho kinase 2
- rho-associated protein kinase 2
- Rho-associated, coiled-coil containing protein kinase 2
- ROCK2
- ROCK-II
- ROKalpha
Background
The small GTPase Rho is implicated in cytoskeletal rearrangements including stress fiber and focal adhesion formation and in the transcriptional activation of c-fos serum response element. In vitro, Rho-kinase, which is activated by Rho, phosphorylates not only myosin light chain (MLC) (thereby activating myosin ATPase) but also myosin phosphatase, thus inactivating myosin phosphatase. Rho-kinase is involved in the formation of stress fibers and focal adhesions in fibroblasts.