Product: 7beta-Hydroxylathyrol
Caveolin-1 Antibody (4C1) Summary
| Immunogen |
Recombinant human CAV1 (1-104aa) purified from E. coli
|
| Marker |
Caveolae Marker, Endosome Marker
|
| Specificity |
CAV1 mAb, clone 4C1, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PTS protein.
|
| Isotype |
IgG2b Kappa
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| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CAV1
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
The antibody has been tested by ELISA, Western blot to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 1000. Recommended starting dilution is 1:500 Application: ELISA, WB
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4) and 10% Glycerol
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein G purified
|
Alternate Names for Caveolin-1 Antibody (4C1)
- CAV
- CAV1
- caveolae protein, 22kD
- caveolin 1, caveolae protein, 22kDa
- Caveolin1
- Caveolin-1
- cell growth-inhibiting protein 32
- MSTP085
- VIP21
Background
The identification of caveolin-1 (CAV1) as the main structural component of caveolae, together with the finding that CAV1 might serve as a molecular organizer for membrane multiprotein complexes involved in cellular traffic, endo-and transcytosis, cell adhesion and signal transduction prompted a new impulse in the research on these intracellular organells. One of the properties of CAV1 was its insolubility in cold non-ionic detergents together with apical markers in epithelial cell. Also, cholesterol is essential for caveolae formation and maintenance, and caveolae structure is highly sensitive to cholesterol depletion or treatment with cholesterol binding drugs. CAV1 tightly and specifically binds free cholesterol and artificial cholesterol containing vesicles.