PSTAIR Antibody (PSTAIR) Summary
| Immunogen |
Synthetic peptide: EGVPSTAIREISLLKE conjugated to BSA.
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| Epitope |
Recognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3).
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| Specificity |
PSTAIR (PSTAIR)
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Purity |
Unpurified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2; when it is complexed with cyclin B.WB: Use at a dilution of 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
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Reactivity Notes
Cross-reacts with human, monkey, mouse, Japanese quail, fish (goldfish, carp, eel, starfish, and amago salmon), Xenopus, cliliates, and plants (lily and onion). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
Raw ascites
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| Preservative |
0.1% Sodium Azide
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| Concentration |
This product is unpurified. The exact concentration of antibody is not quantifiable.
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| Purity |
Unpurified
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Alternate Names for PSTAIR Antibody (PSTAIR)
- EGVPSTAIREISLLKE sequence
- EGVPSTAIREISLLKE
- PSTAIR sequence
Background
The cell cycle is controlled in part by cyclin-dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.