GPR109B/HM74 Antibody Summary
| Immunogen |
Synthetic 18 amino acid peptide from N-terminal extracellular domain of human GPR109B / HM74.
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| Localization |
Multi-pass membrane protein
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| Specificity |
Human GPR109B / HM74. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HCAR2 (100%).
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| Predicted Species |
Primate (100%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
HCAR3
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
ICC/IF usage is reported in scientific literature (PMID: 17008871).
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Gorilla (100%), Panda (89%). Mouse reactivity reported in scientific literature (PMID: 17008871).
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.1% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for GPR109B/HM74 Antibody
- G protein-coupled receptor 109B
- GPR109B
- G-protein coupled receptor 109B
- G-protein coupled receptor HM74
- G-protein coupled receptor HM74B
- GTP-binding protein
- HCA3
- HCAR3
- HM74
- HM74B
- HM74Puma-g
- Niacin receptor 2
- NIACR2
- Nicotinic acid receptor 2
- PUMAG
- putative chemokine receptor
Background
HM74 acts as a low affinity receptor for nicotinic acid and mediates its anti-lipolytic effect through a G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Expression in humans is largely restricted to adipose tissue and spleen but there are some reports of it being expressed in lymphocytes and monocytes. In mouse is expressed in lungs, spleen, heart, skeletal muscle and adipose tissue. In rat it is expressed in adipose tissue, lung and spleen. ESTs have been isolated from adipose, blood, liver, liver/spleen, placenta, prostate, and testis libraries.