AlphaA Crystallin/CRYAA Antibody Summary
| Immunogen |
Purified alpha-A Crystallin from bovine lens.
|
| Localization |
Cytoplasmic
|
| Specificity |
This does not detect the unphosphorylated form of the protein.
|
| Isotype |
IgG
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
CRYAA
|
| Purity |
Immunogen affinity purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
WB: Detects an approx. 20 kDa representing alpha-A crystallin from bovine lens samples. IHC: Staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells.
|
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS and 30 mg/ml BSA
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
LYOPH
|
| Purity |
Immunogen affinity purified
|
| Reconstitution Instructions |
Reconstitute to desired concentration.
|
Alternate Names for AlphaA Crystallin/CRYAA Antibody
- AlphaA Crystallin
- CRYA1
- CRYA1crystallin, alpha-1
- CRYAA
- crystallin, alpha A
- Heat shock protein beta-4
- HSPB4
- HSPB4alpha-crystallin A chain
- human alphaA-crystallin (CRYA1), 10HspB4
Background
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products–alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexanders disease where it accumulates in brain cells of those afflicted.