COPB2 Antibody Summary
| Immunogen |
Synthetic peptide corresponding to residues K(494) V L A A Q E T H E G V T E D G I E(511) of rat beta-COP.
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| Specificity |
Detects coatomer-protein I beta (COP I beta).
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| Isotype |
IgG
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
COPB2
|
| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
ICC/IF usage was reported in scientific literature (PMID: 11056392). WB: Detects an approx. 115 kDa protein representing COP I beta from PC-12 cell extract.
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Reactivity Notes
Human reactivity reported in scientific literature (PMID: 23845592).
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS with 1 mg/ml BSA
|
| Preservative |
0.05% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
|
Alternate Names for COPB2 Antibody
- beta prime subunit
- Beta-coat protein
- Beta-COP
- betaprime-COP
- coatomer protein complex, subunit beta 2 (beta prime)
- coatomer subunit beta
- p102
Background
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP I) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.