IL-1 RAcP/IL-1 R3 Antibody

Product: IDO-IN-2

IL-1 RAcP/IL-1 R3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IL1RAP. The exact sequence is proprietary.
Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein; Isoform 2: Secreted; Isoform 3: Secreted
Predicted Species
Monkey (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL1RAP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (99%), Rat (87%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IL-1 RAcP/IL-1 R3 Antibody

  • C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3
  • FLJ37788
  • IL-1 R3
  • IL-1 RAcP
  • IL-1 receptor accessory protein
  • IL-1R3
  • IL-1R-3
  • IL1RAcP
  • IL-1RAcP
  • IL1RAP
  • interleukin 1 receptor accessory protein
  • interleukin-1 receptor accessory protein beta
  • interleukin-1 receptor accessory protein

Background

Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes an interleukin 1 receptor accessory protein. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. [provided by RefSeq]

PMID: 21957243

IL-1 RAcP/IL-1 R3 Antibody

Product: ONO-4059 (analog)

IL-1 RAcP/IL-1 R3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:CRCCVTYCEGENHLRNKSRAEIHNQPQWETHLCKPVPQESETQWIQNGTRLEPPAPQISALALHHFTDLSNNNDF
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL1RAP
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 – 1:200
Application Notes
For HIER pH6 retrieval is recommended.
Control Peptide
IL-1 RAcP/IL-1 R3 Protein (NBP1-86793PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IL-1 RAcP/IL-1 R3 Antibody

  • C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3
  • FLJ37788
  • IL-1 R3
  • IL-1 RAcP
  • IL-1 receptor accessory protein
  • IL-1R3
  • IL-1R-3
  • IL1RAcP
  • IL-1RAcP
  • IL1RAP
  • interleukin 1 receptor accessory protein
  • interleukin-1 receptor accessory protein beta
  • interleukin-1 receptor accessory protein

PMID: 25392162

IL-1 RAcP/IL-1 R3 Antibody

Product: Ginsenoside Rg6

IL-1 RAcP/IL-1 R3 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 22 amino acids near the carboxy terminus of human IL-1RAcP. The immunogen is located within the last 50 amino acids of IL-1RAcP.
Specificity
It has no cross activity to other member in the IL-1 receptor family.
Clonality
Polyclonal
Host
Rabbit
Gene
IL1RAP
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
IL-1RAcP antibody can be used for detection of IL-1RAcP by Western blot at 1:500 to 1:1000 dilution. An approximately 66 kDa band can be detected. It has no cross activity to other member in the IL-1 receptor family.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
IL-1 RAcP/IL-1 R3 Peptide (NBP1-76924PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IL-1 RAcP/IL-1 R3 Antibody

  • C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3
  • FLJ37788
  • IL-1 R3
  • IL-1 RAcP
  • IL-1 receptor accessory protein
  • IL-1R3
  • IL-1R-3
  • IL1RAcP
  • IL-1RAcP
  • IL1RAP
  • interleukin 1 receptor accessory protein
  • interleukin-1 receptor accessory protein beta
  • interleukin-1 receptor accessory protein

Background

The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kappaB inducing kinae (NIK) and of NF-kappaB, recruites to the IL-1R complex through IL-1 RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

PMID: 23700486