CD14 Antibody (UCH-M1) Summary
| Immunogen |
Thymocytes and peripheral blood leucocytes (PBL)
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| Localization |
Cytoplasmic. Attached to the membrane by a GPI anchor.
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| Isotype |
IgG2a
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
CD14
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
UCH-M1 activates complement.
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| Theoretical MW |
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for CD14 Antibody (UCH-M1)
- CD14 antigen
- CD14 molecule
- CD14
- monocyte differentiation antigen CD14
- Myeloid cell-specific leucine-rich glycoprotein
Background
CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.