PASD1 Antibody (2ALCC128) Summary
| Immunogen |
GST-PASD1 recombinant protein containing the 540-773 aas of PASD1, a region only present in PASD1b.
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| Specificity |
PASD1-2 is specific for the recombinant protein (aa 540-773) present only in the longer PASD1_v2 protein (C-terminus). Antibody PASD1-2 stains nuclei of a subpopulation of spermatogonia near the basal membrane in testicular tubules, with decreasing intensity of labeling with maturity, and also labels a small subpopulation of salivary gland cells. IHC stainings may be performed on FFPE and cryostat preparations of cells and tissues.In WB, expected MW is 87.5 kDa/72.5 kDa, observed MW is ~100 kDa.Specific for PASD1b.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
PASD1
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| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Theoretical MW |
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein G purified
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Notes
Positive control(s): PASD-1 transfected cells and testis; BT-20 and NCI-H1299 carcinoma lines (negative control: tonsil).
Alternate Names for PASD1 Antibody (2ALCC128)
- CT63Cancer/testis antigen 63
- OXTES1
- OX-TES-1
- PAS domain containing 1
- PAS domain-containing protein 1