Cytokeratin 8 Antibody (35betaH11) Summary
| Immunogen |
Clone 35betaH11 is a mouse monoclonal IgM antibody derived by fusion of NS-1 mouse myeloma cells with spleen cells from a Balb/c mouse immunized with a cytoskeletal preparation of the human hepotocellular carcinoma cell line Hep3B.
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| Marker |
Epithelial Cell Marker
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| Isotype |
IgM
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
KRT8
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
35BetaH11 is reactive on frozen tissue sections after fixation in methanol. It is particularly effective on paraffin-embedded tissues after microwave pretreatment of the slides. In addition, 35BetaH11 is suitable for Immunocytochemistry, Immunoblotting. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:500 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Although not tested this antibody may be useful in and Flow cytometry.
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Reactivity Notes
Human
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for Cytokeratin 8 Antibody (35betaH11)
- CARD2
- CK8
- CK-8
- CYK8cytokeratin 8
- Cytokeratin 8
- Cytokeratin-8
- EndoA
- K2C8
- K8cytokeratin-8
- keratin 8
- keratin, type II cytoskeletal 8
- keratin-8
- KO
- KRT8
- Type-II keratin Kb8