Plakophilin-3 Antibody (23E3/4) [DyLight 405] Summary
| Immunogen |
Clone 23E3/4 is a mouse monoclonal IgG2b antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to amino acid residues 570-589 of human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).
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| Isotype |
IgG2b
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
PKP3
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Clone 23E3/4 is suitable for Immunocytochemistry (methanol- and paraformaldehyde-fixed cells), Immunohistochemistry on frozen and paraffin embedded tissues, Immunoprecipitation and Western blot detection. For optimal results, fixed cells and frozen tissues should be treated with 0.2% Triton-X 100 for, respectively, 5 min and 15 min prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
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Reactivity Notes
Human, Mouse and Canine. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
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| Buffer |
50mM Sodium Borate
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| Preservative |
0.05% Sodium Azide
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| Purity |
Protein A or G purified
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Alternate Names for Plakophilin-3 Antibody (23E3/4) [DyLight 405]
- plakophilin 3
- plakophilin-3