ERp57/PDIA3 Antibody Summary
| Immunogen |
Peptide with sequence C-EEKPKKKKKAQEDL corresponding to C-Terminus according to NP_005304.3.
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| Localization |
Endoplasmic reticulum
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| Marker |
Endoplasmic Reticulum Marker
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
PDIA3
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Approx. 55 kDa band observed in lysates of HepG2 and NIH3T3 (calculated MW of 56.8 kDa band according to NP_005304.3).
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| Positive Control |
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Rat, Bovine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for ERp57/PDIA3 Antibody
- Disulfide isomerase ER-60
- EC 5.3.4.1
- endoplasmic reticulum P58
- Endoplasmic reticulum resident protein 57
- Endoplasmic reticulum resident protein 60
- ER protein 60
- ER60
- ERp57
- ERp5758 kDa glucose-regulated protein
- ERp60
- ERp6058 kDa microsomal protein
- ERp61
- glucose regulated protein, 58kDa
- GRP57
- GRP58
- GRP58ER protein 57
- HsT17083
- P58
- PDIA3
- phospholipase C-alpha
- PI-PLC
- protein disulfide isomerase family A, member 3
- protein disulfide isomerase-associated 3
- protein disulfide-isomerase A3
Background
ERp57, also known as GRP58, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.