EphB1 [p Tyr317] Antibody Summary
| Immunogen |
Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of Chicken EphrinB. Note: Chicken Tyr317 is the homolog of rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310.
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| Modification |
p Tyr317
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| Specificity |
Specific for the ~46k EphrinB protein phosphorylated at Tyr317. The immunolabeling of the EphrinB band is blocked by treatment with lambda-phosphatase.
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| Predicted Species |
Human (100%), Xenopus (100%), Bovine (100%), Chicken (100%), Zebrafish (100%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
EPHB1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody has been tested in Western blot on rat lysate, detecting a band ~46 kDa. Immunofluorescence (PMID: 21530605)
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| Publications |
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Reactivity Notes
Tested in Rat. Mouse reactivity reported in scientific literature (PMID: 21539827).
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
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| Preservative |
No Preservative
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| Purity |
Immunogen affinity purified
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Alternate Names for EphB1 [p Tyr317] Antibody
- Cek6
- EC 2.7.10
- EC 2.7.10.1
- EK6
- Elk
- EPH receptor B1
- eph tyrosine kinase 2
- EphB1
- EPH-like kinase 6
- ephrin type-B receptor 1
- EPHT2FLJ37986
- Hek6
- Net
- NETHek6
- soluble EPHB1 variant 1
- Tyrosine-protein kinase receptor EPH-2
Background
EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)