beta-III Tubulin Antibody (AA10) Summary
| Immunogen |
: Synthetic peptide corresponding to beta III tubulin.
|
| Marker |
Neuronal Marker
|
| Specificity |
Specific for the ~55kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1.
|
| Isotype |
IgG
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
TUBB3
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
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Reactivity Notes
This antibody has been directly tested for reactivity in rat. It is expected that this antibody will have wide species cross reactivity.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
|
| Preservative |
No Preservative
|
| Purity |
Protein G purified
|
Alternate Names for beta-III Tubulin Antibody (AA10)
- beta-4
- betaIII Tubulin
- beta-III Tubulin
- beta-Tubulin III
- TUBB4 CFEOM3A
- Tubulin Beta 3
- tubulin beta-3 chain
- Tubulin beta-4 chain
- Tubulin beta-4
- Tubulin beta-III
- tubulin, beta 3
- TUJ1 antigen
- TUJ-1 antigen
Background
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2