IL-27 Antibody (355028) Summary
| Immunogen |
Mouse myeloma cell line NS0-derived recombinant mouse IL-27 heterodimer
Tyr19-Pro228 (EBI3) and Phe29-Ser234 (p28) Accession # O35228 (EBI3) & Q8K3I6 (p28) |
| Specificity |
Detects mouse IL-27 heterodimer in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 p28, rmIL‑27 EBI‑3, or recombinant human (rh) IL-27 heterodimer is observed. In Western blots, no cross-reactivity with rmIL-27 p28 or rhIL-27 heterodimer is observed.
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| Source |
N/A
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| Isotype |
IgG2a
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| Clonality |
Monoclonal
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| Host |
Rat
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| Gene |
IL27
|
| Purity |
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions
| Dilutions |
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Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
|
| Preservative |
No Preservative
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| Concentration |
LYOPH
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| Purity |
Protein A or G purified from hybridoma culture supernatant
|
| Reconstitution Instructions |
Sterile PBS to a final concentration of 0.5 mg/mL.
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Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IL-27 Antibody (355028)
- IL-27 p28 subunit
- IL-27 subunit alpha
- IL27
- IL-27
- IL27A
- IL-27-A
- IL27-A
- IL27p28
- IL30
- interleukin 27
- interleukin 30
- interleukin-27 subunit alpha
- MGC71873
- p28IL-27A
Background
Interleukin 27 (IL-27) is a secreted heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus‑induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naïve CD4+ T cells and induces the expression of a functional IL-12 receptor, allowing IL-12-induced Th1 polarization. Human EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide and a 209 aa mature protein with two fibronectin type III domains.