Angiopoietin-3 Antibody (113538) Summary
| Immunogen |
E. coli-derived recombinant mouse Angiopoietin‑3
Ala21-Ala509 Accession # Q9WVH6 |
| Specificity |
Detects mouse Angiopoietin‑3 in direct ELISAs and Western blots.
|
| Source |
N/A
|
| Isotype |
IgG2a
|
| Clonality |
Monoclonal
|
| Host |
Rat
|
| Gene |
Angpt4
|
| Purity |
Protein A or G purified from hybridoma culture supernatant
|
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Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
|
| Preservative |
No Preservative
|
| Concentration |
LYOPH
|
| Purity |
Protein A or G purified from hybridoma culture supernatant
|
| Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS.
|
Notes
Alternate Names for Angiopoietin-3 Antibody (113538)
- Angiopoietin-3
- ANGPT3
Background
Mouse Angiopoietin‑3 (ANG-3) (1), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Mouse ANG-3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. It shares 47%, 46% and 54% aa sequence identity with mouse ANG-1, mouse ANG-2 and human ANG-4, respectively. Although the sequence homology is much higher between the human and mouse counterparts for ANG-1 (97%) and ANG-2 (85%), mouse ANG-3 is believed to be an ortholog of human ANG-4 based on chromosomal localization studies (1, 2). Human ANG-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse ANG-3 is expressed in multiple mouse tissues. Human ANG-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse ANG-3 has been reported to be a Tie-2 antagonist. It is likely that mouse ANG-3, like ANG-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).