Pax6 Antibody (PAX6/1166) – IHC-Prediluted Summary
| Immunogen |
Recombinant fragment (N-terminus; aa 1-300) of human PAX6 protein
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| Localization |
Nuclear
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| Marker |
Stem Cell Marker
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| Specificity |
Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.
|
| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
|
| Gene |
PAX6
|
| Purity |
Protein A or G purified
|
| Innovators Reward |
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 11p13 Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for Pax6 Antibody (PAX6/1166) – IHC-Prediluted
- keratitis)
- MGC17209
- Oculorhombin
- paired box 6
- paired box protein Pax-6
- Pax6