Chicken anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)

Product: Tyrphostin A10

Chicken anti-Mouse IgG (H L) Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Purified Mouse IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, rabbit IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Readout System
  • NovaLume Plus Chemiluminescent Reagent

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C. After reconstitution, dilute with 50% glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.5 ml deionized water.

Notes

Purity > 95% based on SDS-PAGE. Ig fraction was prepared using eggs obtained from healthy hens of US origin. After reconstituted, prepare fresh working dilution daily.

PMID: 15854203