LD78 beta Antibody Summary
| Immunogen |
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 70 amino acids of the mature swine CCL3L1 protein.
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| Specificity |
This antibody is specific for swine CCL3L1 protein. A BLAST analysis was used to suggest cross-reactivity with CCL3L1 from bovine sources based on 100% identity with the immunizing sequence. Partial reactivity is expected against CCL3L1 from cat, dog, human, chimpanzee, baboon, bovine, and rat based on greater than 90% sequence homology. Cross-reactivity with CCL3L1 from other sources has not been determined.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
CCL3L1
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| Purity |
Protein A purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This protein-A purified antibody has been tested for use in ELISA and western blotting.Expect a band approximately 7.8 kDa in size corresponding – swine CCL3L1 protein by western blotting in the appropriate cell lysate or extract.
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Reactivity Notes
swine and bovine
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.01% Sodium Azide
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| Concentration |
LYOPH
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| Purity |
Protein A purified
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| Reconstitution Instructions |
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.
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Alternate Names for LD78 beta Antibody
- C-C motif chemokine 3-like 1
- CCL3L3
- chemokine (C-C motif) ligand 3-like 1
- D17S1718
- G0/G1 switch regulatory protein 19-2
- G0S19-2LD78
- LD78BETA
- LD78-beta(1-70)
- MGC104178
- MGC12815
- MGC182017
- MIP1AP
- PAT 464.2
- SCYA3L
- SCYA3L1464.2
- small inducible cytokine A3-like 1
- Small-inducible cytokine A3-like 1
- Tonsillar lymphocyte LD78 beta protein
Background
Chemokine (C-C motif) ligand 3-like 1 (CCL3L1), also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha; other synonyms: ld78beta, scya3l1, small inducible cytokine a3-like 1), is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. In humans, CCL3L1 is encoded by a variable copy-number gene. The CC family induces MCP1 and RANTES, and exhibits a variety of proinflammatory activities including chemotaxis, and functional and proliferative activation of leukocytes, lymphocytes, and macrophages. Its signal is transmitted through transmembrane receptors, CC chemokine receptors, CCR1, CCR3 and CCR5.
CCL3L1/MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils. CCL3L1 binds to several chemokine receptors. In humans these receptors include chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. In swine, CCL3L1 protein is 86% similar to CCL4 protein, and 73% similar to CCL5.
CCL3L1/MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils. CCL3L1 binds to several chemokine receptors. In humans these receptors include chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. In swine, CCL3L1 protein is 86% similar to CCL4 protein, and 73% similar to CCL5.