Donkey anti-Mouse IgG (H L) Secondary Antibody [DyLight 550] Summary
| Immunogen |
Purified Mouse IgG (H&L)
|
| Specificity |
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins. No reactivity is observed to non-immunoglobulin mouse serum immunoglobulins.
|
| Isotype |
IgG
|
| Clonality |
Polyclonal
|
| Host |
Donkey
|
| Purity |
Immunogen affinity purified
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Antibody is > 95% based on SDS-PAGE.
|
Packaging, Storage & Formulations
| Storage |
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
|
| Buffer |
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
LYOPH
|
| Purity |
Immunogen affinity purified
|
| Reconstitution Instructions |
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
|
Notes
Affinity purified antibody is > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 550 (Ex = 550 nm; Em = 576 nm)
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.