Thymidylate Synthase Antibody (TMS715) [HRP]

Product: HOE 33188

Thymidylate Synthase Antibody (TMS715) [HRP] Summary

Immunogen
Recombinant human thymidylate synthase
Localization
Nuclear & Cytoplasmic
Marker
5-FU Resistance Marker
Specificity
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TYMS
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
  • NovaLume Plus Chemiluminescent Reagent

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for Thymidylate Synthase Antibody (TMS715) [HRP]

  • EC 2.1.1.45
  • HsT422
  • MGC88736
  • thymidylate synthase
  • thymidylate synthetase
  • TMSTsase
  • TSase
  • TSHST422

PMID: 17690692