Kappa Light Chain Antibody (HP6053) – Azide and BSA Free

Product: AG-1479

Kappa Light Chain Antibody (HP6053) – Azide and BSA Free Summary

Immunogen
Purified human Ig kappa chain
Localization
Cell Surface, cytoplasmic and secreted
Marker
B-Cell Marker
Specificity
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGKC
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-2.0ug/million cells
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
  • CyTOF-ready
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS – BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Kappa Light Chain Antibody (HP6053) – Azide and BSA Free

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

PMID: 19466990