IgG Antibody (SPM566) – Azide and BSA Free

Product: Parecoxib (Sodium)

IgG Antibody (SPM566) – Azide and BSA Free Summary

Immunogen
Purified human Ig Gamma Chain
Localization
Cytoplasm, Cell Surface and Secreted
Marker
B Cell Marker
Specificity
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHG1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug
  • CyTOF-ready
Application Notes
Immunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS – BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for IgG Antibody (SPM566) – Azide and BSA Free

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

PMID: 2542048