Cyclin B1 Antibody (V92.1) Summary
| Immunogen |
Hamster Cyclin B1 protein
|
| Localization |
Cytoplasmic
|
| Specificity |
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
|
| Isotype |
IgG1 Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CCNB1
|
| Purity |
Protein A purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
ELISA (For coating, order NBP2-34509 (BSA free version of NBP2-34312))
|
| Theoretical MW |
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
PBS with 0.05% BSA.
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A purified
|
Alternate Names for Cyclin B1 Antibody (V92.1)
- CCNB
- CCNB1
- Cyclin B1
- G2/mitotic-specific cyclin B1
- G2/mitotic-specific cyclin-B1