MUC-1 Antibody (SPM132) – IHC-Prediluted Summary
| Immunogen |
Human milk fat globule membranes
|
| Localization |
Cytoplasmic and cell surface
|
| Marker |
Epithelial Marker
|
| Specificity |
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
|
| Isotype |
IgG1 Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
MUC1
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 1q22
Molecular weight of antigen: 265-400kDa Immunohistochemistry-Paraffin 0. 1 – 0.2 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for MUC-1 Antibody (SPM132) – IHC-Prediluted
- Breast carcinoma-associated antigen DF3
- Carcinoma-associated mucin
- CD227 antigen
- CD227
- DF3 antigen
- EMA
- Episialin
- H23 antigen
- H23AG
- KL-6
- MAM6
- MUC-1
- MUC1/ZD
- mucin 1, cell surface associated
- mucin 1, transmembrane
- Mucin-1
- Peanut-reactive urinary mucin
- PEM
- PEMMUC-1/SEC
- PEMT
- Polymorphic epithelial mucin
- PUMMUC-1/X
- tumor associated epithelial mucin
- Tumor-associated epithelial membrane antigen
- Tumor-associated mucin