Donkey anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase]

Product: Primidone

Donkey anti-Rabbit IgG (H L) Secondary Antibody [Alkaline Phosphatase] Summary

Immunogen
Purified Rabbit IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.

Packaging, Storage & Formulations

Storage
Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Purity > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

PMID: 10478637