Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [FITC]

Product: Methscopolamine (bromide)

Goat F(ab)2 anti-Rabbit IgG (H L) Secondary Antibody [FITC] Summary

Immunogen
Purified Rabbit IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with bovine, human, or mouse IgG or serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20-1:2000
  • Immunomicroscopy 1:20-1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Reactivity Notes

Based on IEP, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Purity > 90% based on SDS-PAGE Small amounts of intact IgG may be present.. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

PMID: 23667248

Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [FITC]

Product: Anamorelin (Fumarate)

Goat F(ab)2 anti-Rabbit IgG (H L) Secondary Antibody [FITC] Summary

Immunogen
Purified Rabbit IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20-1:2000
  • Immunomicroscopy 1:20-1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Packaging, Storage & Formulations

Storage
Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Purity > 90% based on SDS-PAGE Small amounts of intact IgG may be present.. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

PMID: 17437993

Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [FITC]

Product: Isoginkgetin

Goat F(ab)2 anti-Rabbit IgG (H L) Secondary Antibody [FITC] Summary

Immunogen
Goat Anti-Rabbit IgG (H+L)-FITC Conjugate
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
The antibody conjugate has been tested by FACS analysis at 0.75 ug/one million cells to assure specificity and reactivity. Since applications vary, investigators should determine dilutions appropriate for individual use.

Reactivity Notes

Minimal Cross Reactivity: Human, Mouse, Rat Serum Proteins

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative
0.05% Sodium Azide
Concentration
1.5 mg/ml
Purity
Immunogen affinity purified

Background

Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Minimal cross-reaction to human, bovine, horse and rabbit.

PMID: 17329551