IPO-38 Antibody (IPO-38) Summary
| Immunogen |
Spleen cells of a patient with hairy cell leukemia
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| Localization |
Nuclear
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| Marker |
Proliferation Marker
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| Specificity |
Recognizes a protein of 14-16kDa, which is a novel nuclear antigen of proliferating cells. IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO-38 may be a useful marker of cell proliferation during monitoring of tumor progression.
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| Isotype |
IgM Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Molecular weight of antigen: 14-16kDa
Immunohistochemistry-Paraffin 1:25 – 1:50 for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
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| Buffer |
10mM PBS and 0.05% BSA
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| Preservative |
0.05% Sodium Azide
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| Purity |
Protein A or G purified
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