Fibronectin Antibody (568) – IHC-Prediluted Summary
| Immunogen |
High molecular weight proteins secreted by cultivated human fibroblasts
|
| Localization |
Connective tissue matrix
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| Specificity |
Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.
|
| Isotype |
IgG1 Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
FN1
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 2q35 Molecular weight of antigen: 220kDa (monomer); 440kDa (dimer) Immunohistochemistry-Paraffin 1 – 2 ug/ml for 30-60 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 – 20 min followed by cooling at RT for 20 minutes.
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Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for Fibronectin Antibody (568) – IHC-Prediluted
- Fibronectin
- FN1