Fibronectin Antibody (568)

Product: Bruceine A

Fibronectin Antibody (568) Summary

Immunogen
High molecular weight proteins secreted by cultivated human fibroblasts
Localization
Connective tissue matrix
Specificity
Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 – 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 1 – 2 ug/ml
  • Immunofluorescence 0.5 – 1.0 ug/ml
Application Notes
Hu-chromosome location: 2q35
Molecular weight of antigen: 220kDa (monomer); 440kDa (dimer)
Immunohistochemistry-Paraffin 1 – 2 ug/ml for 30-60 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 – 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Fibronectin Antibody (568)

  • Fibronectin
  • FN1

PMID: 20653956