Product: Demeclocycline (hydrochloride)
NF-L Antibody (SPM204) – IHC-Prediluted Summary
| Immunogen |
Neurofilament purified from human brain
|
| Localization |
Cytoplasmic
|
| Marker |
Neuronal Marker
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| Specificity |
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
NEFL
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 8p21.2 Immunohistochemistry-Paraffin 0.25 – 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for NF-L Antibody (SPM204) – IHC-Prediluted
- 68 kDa neurofilament protein
- CMT2E
- NEFL
- neurofilament protein, light chain
- neurofilament subunit NF-L
- Neurofilament triplet L protein
- neurofilament, light polypeptide
- neurofilament-light
- NF68
- NF68FLJ53642
- NFL
- NF-L
- NFLlight polypeptide 68kDa