S100A9 Antibody (CPT/1028) Summary
| Immunogen |
Recombinant human Calprotectin protein
|
| Localization |
Cytoplasmic
|
| Marker |
Macrophage Marker
|
| Specificity |
Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain. Calprotectin comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and is important for identifying macrophages in tissue sections.
|
| Isotype |
IgM Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
S100A9
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 1q21
Not suitable for Immunohistochemistry-Frozen tissues. Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
1.0mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
0.2 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for S100A9 Antibody (CPT/1028)
- CAGBMigration inhibitory factor-related protein 14
- Calgranulin B
- calgranulin-B
- Calprotectin L1H subunit
- CFAGMRP-14,60B8AG
- CGLB
- L1AG
- LIAG
- MAC387
- MIF
- MRP-14
- MRP14Leukocyte L1 complex heavy chain
- NIF
- P14
- protein S100-A9
- S100 calcium binding protein A9 (calgranulin B)
- S100 calcium binding protein A9
- S100 calcium-binding protein A9 (calgranulin B)
- S100 calcium-binding protein A9
- S100A9