Nitrotyrosine Antibody (EM-30) Summary
| Immunogen |
NO2-Tyr-CH2-Thyroglobulin
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| Specificity |
The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
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| Host |
Mouse
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody I useful for Western Blot (Positive control:NO2-BSA, NO2-HS) Immunohistochemistry-paraffin( Positive tissue: colon cancer epithelium, prostate hyperplasia)and Immunohistochemistry-frozen (Positive tissue: colon cancer epithelium, prostate hyperplasia)
|
Reactivity Notes
This antibody reacts to large number of species.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS (pH 7.4)
|
| Preservative |
15mM Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Immunogen affinity purified
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Alternate Names for Nitrotyrosine Antibody (EM-30)
- Nitrotyrosine
Background
Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.