Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [HRP]

Product: CAY10604

Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [HRP] Summary

Immunogen
Fusion protein of human IgG Fc fragment.
Specificity
The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
Application Notes
This antibody is useful in Western Blot, Immunocytochemistry, ELISA.
Readout System
  • NovaLume Plus Chemiluminescent Reagent

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 1.0% (w/v) high grade BSA (protease free)
Preservative
0.01% Thimerosal
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

PMID: 12065755