Mouse anti-Human IgG Fc Secondary Antibody (EM-07) Summary
| Immunogen |
Fusion protein of human IgG Fc fragment.
|
| Specificity |
The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Purity |
Protein A purified
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western Blot and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB. The antibody EM-07 does not crossreact with IgM.
|
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS (pH 7.4)
|
| Preservative |
15mM Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein A purified
|
Background
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.