Lightning-Link Rapid FluoProbes647H Antibody Labeling Kit

Product: Fluo-4AM

Lightning-Link Rapid FluoProbes647H Antibody Labeling Kit Summary

Description
Lightning-Link Rapid is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.

The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates (for more details please watch the video below).

Features Benefits
Quick and easy to use Save time, no special knowledge required
No separation steps 100% recovery – no antibody/protein loss
Can be used in a wide range of applications Flexible
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more) Easy transfer from R&D to manufacturing
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 references Successfully used in many fields of research

FluoProbes647H is one of a new generation of fluorescent labels designed to label biomolecules. It has a strong absorption at 650nm, high fluorescence at 684nm (extinction coefficient 2.5 x105 cm-1M-1) and high quantum yield.

Popular conjugates applications:
1. Western Blot
2. Immunofluorescence
3. Immunohistochemistry
4. Flow Cytometry
5. ELISA
6. FRET

Kit Type
Antibody Labeling Kit

Applications/Dilutions

Application Notes
The technology is fully scalable (ug to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.
Publications
Read Publication using 362-0015.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. 1 vial of LL-Rapid Modifier reagent
  2. 1 vial of LL-Rapid Quencher reagent
  3. 1 or 3 glass vial(s) of Lightning-Link Rapid mix

Notes

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Fluorescent Labels Poster
Antibody Labeling Guide
Antibody Purification Guide

Lightning Link® is a registered trademark of Innova Biosciences.

PMID: 11704975