Recombinant Human VEGF 165 Protein Summary
| Description |
Recombinant human VEGF 165 is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture. Endotoxin level is less than 0.005ng per ug of product (0.05EU/ug) as measured by turbidimetric kinetic assay.
|
| Preparation Method |
Produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
|
| Details of Functionality |
The bioactivity recombinant human VEGF 165 was determined by its dose dependent effect on proliferation of Human Umbilical Vein Endothelial Cells (HUVEC). The ED50 for this effect is typically below 10 ng/ml corresponding to specific activity of > 1 x 10e5 U/mg.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
VEGFA
|
| Endotoxin Note |
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kinetic assay.
|
Applications/Dilutions
| Application Notes |
Recombinant human VEGF165 contains 165 amino acids and a 16 a.a. Histidine-based tag for a total length of 181 a.a. and has a predicted molecular mass of 21.3 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 30 kDa in SDS-PAGE.
|
|
| Publications |
|
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
From PBS, pH 7.2.
|
| Preservative |
No Preservative
|
| Concentration |
LYOPH
|
| Reconstitution Instructions |
Reconstitute with sterilized water to a final concentration of at least 0.1 mg/ml.
|
Notes
Product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. Purity is greater than 95% by SDS-PAGE gel analysis. After reconstituted, recommend add a carrier protein (0.1% HSA or BSA) for long term storage.
Alternate Names for Recombinant Human VEGF 165 Protein
- MVCD1
- vascular endothelial growth factor A
- Vascular permeability factor
- VEGF-A
- VEGFMGC70609
- VPFvascular endothelial growth factor
Background
VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.