EGF R/ErbB1 Antibody (6F1) Summary
| Immunogen |
Recombinant fragment, corresponding to amino acids 648-1186 of Human EGFR.
|
| Localization |
Cell Membrane and Secreted
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| Specificity |
NB600-1524 recognises human EGFR. This does not recognize human c-erbB2.
|
| Isotype |
IgG2b
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
EGFR
|
| Purity |
Ammonium sulfate precipitation
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
This antibody can be used for staining of cultured cells but cannot be used for paraffin sections. WB dilutions recommended for colorimetric detection of ~170 kDa.
|
Reactivity Notes
Human. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.2), 10 mg/ml Sucrose and 1.0 mg/ml Sodium Azide
|
| Preservative |
Sodium Azide
|
| Concentration |
0.1 mg/ml
|
| Purity |
Ammonium sulfate precipitation
|
Alternate Names for EGF R/ErbB1 Antibody (6F1)
- avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
- cell growth inhibiting protein 40
- cell proliferation-inducing protein 61
- EC 2.7.10
- EC 2.7.10.1
- EGF R
- EGFR
- epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
- epidermal growth factor receptor
- ErbB
- ErbB1
- ERBB1PIG61
- HER1
- HER-1
- mENA
- Proto-oncogene c-ErbB-1
- Receptor tyrosine-protein kinase erbB-1
Background
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.