Human IgM Antibody (ICL-931) Summary
| Immunogen |
purified human IgM. Specificity of the monoclonal was determined by ELISA on purified human immunoglobulin isotypes.
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| Specificity |
This monoclonal antibody reacts with human IgM, mu-chain. No cross reaction with human plasma proteins other then human IgM. No cross reaction with purified human IgA, IgE and IgG. Apparent binding constant is approximately 0.01 nM. Conjugation of the antibody with peroxidase, FITC or biotin does not impair the binding properties. Specificity of the monoclonal was determined by ELISA on purified human immunoglobulin isotypes.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Purity |
Ion exchange chromatography
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody reacts with human IgM in ELISA, RIA and dot-blot. Further useful to detect human IgM on frozen sections and to selectively deplete human IgM from plasma by means of immunoabsorption.In sandwich ELISA application this antibody can be used for coating of plastic using a secondary antibody with selected HRP or FITC as detection label.
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
10mM Sodium Phosphate (pH 7.5), 0.15M NaCl and 1.0% Mannitol
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| Preservative |
No Preservative
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| Concentration |
1.0 mg/ml
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| Purity |
Ion exchange chromatography
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