CD47 Antibody (BRIC126) Summary
| Immunogen |
Human erythrocytes.
|
| Specificity |
Recognizes the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype. Immunogen: Human erythrocytes Cross reactivity: Porcine, canine, ovine, bovine
|
| Isotype |
IgG2b
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CD47
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
The antibody is very useful for flow cytometry (dilution 1/10), immunohistochemistry on frozen sections. With this antibody immunoprecipitations can be pre-formed.For flow cytometry use 10 ul of the suggested working dilution to label 106 cells in 100 ul. Concentration of the antibody is approximately 1.0 mg/ml.
|
Reactivity Notes
May cross react with porcine, canine, ovine, bovine.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
Tris-Glycerine (pH 7.8)
|
| Preservative |
0.09% Sodium Azide
|
| Purity |
Protein G purified
|
Alternate Names for CD47 Antibody (BRIC126)
- antigen identified by monoclonal 1D8
- Antigenic surface determinant protein OA3
- CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
- CD47 antigen
- CD47 glycoprotein
- CD47 molecule
- CD47
- IAP
- IAPintegrin associated protein
- Integrin-associated protein
- leukocyte surface antigen CD47
- MER6integrin-associated signal transducer
- OA3
- Protein MER6
- Rh-related antigen
Background
CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells. OX-101 (this clone) recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).